New England Biolabs Canada
 

Product Pathways - Protein Translation

BiP Blocking Peptide #1084

Item# Description List Price Web Price Qty
1084S BiP Blocking Peptide - 100 µg $472.00
$424.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
3177S BiP (C50B12) Rabbit mAb - 100 µl $383.00
$344.70
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3177T BiP (C50B12) Rabbit mAb - 20 µl $163.00
$146.70
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This product is assembled upon order. Please allow up to three weeks for your product to be processed.

Description

This peptide is used to block BiP (C50B12) Rabbit mAb #3177 reactivity in dot blot and western blot protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks BiP (C50B12) Rabbit mAb #3177 by dot blot.

Directions For Use

Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting and dot blot protocols.

Background

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).

  1. Wabl, M. and Steinberg, C. (1982) Proc Natl Acad Sci U S A 79, 6976-8.
  2. Haas, I.G. and Wabl, M. (1983) Nature 306, 387-9.
  3. Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
  4. Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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