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Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882

Item# Description List Price Web Price Qty
11882S Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) - 100 µl $436.00
$392.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
Application Dilution Species-Reactivity Sensitivity Isotype
IF-F Human, Mouse, Rat Endogenous Rabbit IgG
IF-IC
F

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Protocols

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Ki-67 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Ki-67 protein.

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat colon using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) and Propidium Iodide (PI) /RNase Staining Solution #4087 (DNA content).


Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

  1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
  3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
  4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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