New England Biolabs Canada
 

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser216) Blocking Peptide #1190

Item# Description List Price Web Price Qty
1190S Phospho-cdc25C (Ser216) Blocking Peptide - 100 µg $458.00
$412.20
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
4901S Phospho-cdc25C (Ser216) (63F9) Rabbit mAb - 100 µl $439.00
$395.10
ADD TO CART
4901T Phospho-cdc25C (Ser216) (63F9) Rabbit mAb - 20 µl $174.00
$156.60
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS

This product is assembled upon order. Please allow up to three weeks for your product to be processed.

Description

This peptide is used to block Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 signal in dot blot.

Directions For Use

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Background

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites