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XP Monoclonal Antibody

Product Pathways - Cell Cycle / Checkpoint

Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate) #11917

Item# Description List Price Web Price Qty
11917S Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate) - 100 µl $521.00
$468.90
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
8516S Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb - 100 µl $480.00
$432.00
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8516T Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb - 20 µl $192.00
$99.00
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Application Dilution Species-Reactivity Sensitivity Isotype
F Human, Mouse, Rat, Monkey Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: F=Flow Cytometry

Protocols

Specificity / Sensitivity

Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser807/811 of human Rb protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of BT-549 (blue) and Jurkat (green) cells using Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (PE Conjugate).

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516.

Background

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

  1. Sherr, C.J. (1996) Science 274, 1672-7.
  2. Nevins, J.R. (1992) Science 258, 424-9.
  3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
  4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
  5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
  6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
  7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
  8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
  9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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