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Product Pathways - Protein Stability

ADRM1 (D9Z1U) Rabbit mAb #12019

Item# Description List Price Web Price Qty
12019S ADRM1 (D9Z1U) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 45 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

ADRM1 (D9Z1U) Rabbit mAb recognizes endogenous levels of total ADRM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ADRM1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ADRM1 (D9Z1U) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ADRM1 (hADRM1-Myc/DDK; +), using ADRM1 (D9Z1U) Rabbit mAb.

IP

IP

Immunoprecipitation of ADRM1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ADRM1 (D9Z1U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot detection was performed using ADRM1 (D9Z1U) Rabbit mAb.


Background

Currently, there are five ubiquitin receptors associated with the proteasome: two proteasome subunits, Rpn10/S5a/PSMD4 and Rpn13/ADRM1 (Adhesion-regulating molecule 1), and three families of shuttling factors, Rad23, Dsk2, and Ddi1. ADRM1 is a ubiquitin receptor of the proteasome (1,2) that binds ubiquitin via a pleckstrin homology domain known as the pleckstrin-like receptor for ubiquitin (Pru) domain. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37 (3-5), perhaps serving as a mechanism to accelerate ubiquitin chain disassembly. A murine Adrm1 knockout results in defective gametogenesis, thus highlighting a physiologic role for endogenous ADRM1 in mammalian development (6).

  1. Schreiner, P. et al. (2008) Nature 453, 548-52.
  2. Husnjak, K. et al. (2008) Nature 453, 481-8.
  3. Yao, T. et al. (2006) Nat Cell Biol 8, 994-1002.
  4. Hamazaki, J. et al. (2006) EMBO J 25, 4524-36.
  5. Qiu, X.B. et al. (2006) EMBO J 25, 5742-53.
  6. Al-Shami, A. et al. (2010) PLoS One 5, e13654.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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