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Product Pathways - Protein Translation

PhosphoPlus® PERK (Thr980) Antibody Duet #12185

Item# Description List Price Web Price Qty
12185S PhosphoPlus® PERK (Thr980) Antibody Duet - 1 Kit $735.00
$661.50
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Duet Includes Quantity Applications Reactivity Homology† Mol. Wt. Isotype
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179 100 µl W R Mouse 170 kDa Rabbit IgG
PERK (C33E10) Rabbit mAb #3192 100 µl W H, M, R, Mk 140 kDa Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting

Reactivity Key: R=Rat, H=Human, M=Mouse, Mk=Monkey

Western Blotting

Western Blotting

Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PERK (C33E10) Rabbit mAb.

Protocols

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.


 

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