New England Biolabs Canada

Product Pathways - Protein Translation

PhosphoPlus® PERK (Thr980) Antibody Duet #12185

Item# Description List Price Web Price Qty
12185S PhosphoPlus® PERK (Thr980) Antibody Duet - 1 Kit $735.00
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at
Duet Includes Quantity Applications Reactivity Homology† Mol. Wt. Isotype
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179 100 µl W R Mouse 170 kDa Rabbit IgG
PERK (C33E10) Rabbit mAb #3192 100 µl W H, M, R, Mk 140 kDa Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting

Reactivity Key: R=Rat, H=Human, M=Mouse, Mk=Monkey

Western Blotting

Western Blotting

Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PERK (C33E10) Rabbit mAb.



PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.


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