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Product Pathways - Innate Immunity

NLRC4 (D5Y8E) Rabbit mAb #12421

Item# Description List Price Web Price Qty
12421S NLRC4 (D5Y8E) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 110 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

NLRC4 (D5Y8E) Rabbit mAb recognizes endogenous levels of total NLRC4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu942 of human NLRC4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 and MUTZ-3 cells using NLRC4 (D5Y8E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human NLRC4 (hNLRC4; +), using NLRC4 (D5Y8E) Rabbit mAb.

IP

IP

Immunoprecipitation of NLRC4 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or NLRC4 (D5Y8E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NLRC4 (D5Y8E) Rabbit mAb.


Background

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich-repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7).

The NLRC4 (IPAF) inflammasome forms in response to bacterial flagellin as well as components of the bacterial conserved type II secretion system (TTSS) (8-12). Ligand detection and ligand-dependent NLRC4 oligomerization and inflammasome activation require the NAIP family of proteins (13,14). In mice, NAIP5 and NAIP6 associate with flagellin, while NAIP2 interacts with TTSS rod proteins (13,14). In humans, NAIP recognizes the TTSS needle protein Cprl (14). In addition, NLRC4 is phosphorylated by PKCδ in response to bacterial infection and this phosphorylation is required for inflammasome assembly and caspase-1 activation (15).

  1. Elinav, E. et al. (2011) Immunity 34, 665-79.
  2. Inohara, N. et al. (1999) J Biol Chem 274, 14560-7.
  3. Ogura, Y. et al. (2001) J Biol Chem 276, 4812-8.
  4. Sabbah, A. et al. (2009) Nat Immunol 10, 1073-80.
  5. Mariathasan, S. et al. (2004) Nature 430, 213-8.
  6. Agostini, L. et al. (2004) Immunity 20, 319-25.
  7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.
  8. Poyet, J.L. et al. (2001) J Biol Chem 276, 28309-13.
  9. Mariathasan, S. et al. (2004) Nature 430, 213-8.
  10. Franchi, L. et al. (2006) Nat Immunol 7, 576-82.
  11. Miao, E.A. et al. (2006) Nat Immunol 7, 569-75.
  12. Miao, E.A. et al. (2010) Proc Natl Acad Sci U S A 107, 3076-80.
  13. Kofoed, E.M. and Vance, R.E. (2011) Nature 477, 592-5.
  14. Zhao, Y. et al. (2011) Nature 477, 596-600.
  15. Qu, Y. et al. (2012) Nature 490, 539-42.

Application References

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