Product Pathways - Innate Immunity
NLRC4 (D5Y8E) Rabbit mAb #12421
PhosphoSitePlus® protein, site, and accession data:
NLRC4
| Item# | Description | List Price | Web Price | Qty |
|---|
| Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| W | Human | Endogenous | 110 | Rabbit IgG | |
| IP |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Protocols
Specificity / Sensitivity
NLRC4 (D5Y8E) Rabbit mAb recognizes endogenous levels of total NLRC4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu942 of human NLRC4 protein.
Western Blotting
Western blot analysis of extracts from THP-1 and MUTZ-3 cells using NLRC4 (D5Y8E) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human NLRC4 (hNLRC4; +), using NLRC4 (D5Y8E) Rabbit mAb.
IP
Immunoprecipitation of NLRC4 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or NLRC4 (D5Y8E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NLRC4 (D5Y8E) Rabbit mAb.
Background
The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich-repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7).
The NLRC4 (IPAF) inflammasome forms in response to bacterial flagellin as well as components of the bacterial conserved type II secretion system (TTSS) (8-12). Ligand detection and ligand-dependent NLRC4 oligomerization and inflammasome activation require the NAIP family of proteins (13,14). In mice, NAIP5 and NAIP6 associate with flagellin, while NAIP2 interacts with TTSS rod proteins (13,14). In humans, NAIP recognizes the TTSS needle protein Cprl (14). In addition, NLRC4 is phosphorylated by PKCδ in response to bacterial infection and this phosphorylation is required for inflammasome assembly and caspase-1 activation (15).
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- Mariathasan, S. et al. (2004) Nature 430, 213-8.
- Franchi, L. et al. (2006) Nat Immunol 7, 576-82.
- Miao, E.A. et al. (2006) Nat Immunol 7, 569-75.
- Miao, E.A. et al. (2010) Proc Natl Acad Sci U S A 107, 3076-80.
- Kofoed, E.M. and Vance, R.E. (2011) Nature 477, 592-5.
- Zhao, Y. et al. (2011) Nature 477, 596-600.
- Qu, Y. et al. (2012) Nature 490, 539-42.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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