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XP Monoclonal Antibody

Product Pathways - Protein Folding

Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb #12815

Item# Description List Price Web Price Qty
12815S Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb - 100 µl $433.00
$389.70
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12815T Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb - 20 µl $184.00
$165.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat Endogenous 100 Rabbit IgG
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb recognizes endogenous levels of total prostate specific membrane antigen. An SDS resistant dimer at approximately 200 kDa can be detected depending on sample preparation conditions.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of human prostate specific membrane antigen protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb. Note positive staining of blood vessels.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Prostate Specific Membrane Antigen (D7I8E) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Background

Prostate specific membrane antigen (PSMA, also known as FOLH1), a type II transmembrane protein of the M28 family, has both folate hydrolase and N-acetylated-alpha-linked acidic dipeptidase activity. PSMA was originally identified in the LNCaP cell line, which was derived from a prostate adenocarcinoma lymph node metastasis (1,2). PSMA is an established prostate cancer marker (3); however, it is expressed in other tissues, including kidney, liver, and urinary bladder (4), and it is associated with tumor neovasculature (5) as well. Research studies suggest that PSMA is both a potential diagnostic readout and therapeutic target (6-8).

  1. Horoszewicz, J.S. et al. (1987) Anticancer Res 7, 927-35.
  2. Israeli, R.S. et al. (1993) Cancer Res 53, 227-30.
  3. Ben Jemaa, A. et al. (2010) J Exp Clin Cancer Res 29, 171.
  4. Kinoshita, Y. et al. (2006) World J Surg 30, 628-36.
  5. Chang, S.S. et al. (1999) Mol Urol 3, 313-320.
  6. Mease, R.C. et al. (2013) Curr Top Med Chem 13, 951-62.
  7. Frigerio, B. et al. (2013) Eur J Cancer 49, 2223-32.
  8. Denmeade, S.R. et al. (2012) Sci Transl Med 4, 140ra86.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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