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Product Pathways - Protein Folding

DNAJC2/MPP11 (D6B1E) Rabbit mAb #12844

Item# Description List Price Web Price Qty
12844S DNAJC2/MPP11 (D6B1E) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 80 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

DNAJC2/MPP11 (D6B1E) Rabbit mAb recognizes endogenous levels of total DNAJC2/MPP11 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DNAJC2/MPP11 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DNAJC2/MPP11 (D6B1E) Rabbit mAb.

IP

IP

Immunoprecipitation of DNAJC2/MPP11 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DNAJC2/MPP11 (D6B1E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using DNAJC2/MPP11 (D6B1E) Rabbit mAb.

Background

DnaJ/Hsp40 proteins are a conserved family of J-domain-containing chaperone proteins that assist in protein folding and stability through their interactions with Hsp70 chaperone proteins (reviewed in 1). DNAJC2, also known as MPP11 (M-phase phosphoprotein 11 protein) or ZRF1, is a component of the ribosome-associated complex (RAC). The RAC is localized to the cytoplasm, where it assists in maintaining appropriate folding of nascent polypeptides by stimulating the ATPase activity of Hsp70 chaperone proteins (2,3). In the nucleus, MPP11 is involved in the activation of transcription through mediation of the switch from polycomb-repressed to active chromatin (4). Previous studies have shown MPP11 is overexpressed in leukemia and head and neck cancer, leading researchers to suggest MPP11 may be a potential therapeutic target (5-7). MPP11 is phosphorylated at serine 47 by S6 kinase, which regulates senescence in fibroblast cells (8).

  1. Qiu, X.B. et al. (2006) Cell Mol Life Sci 63, 2560-70.
  2. Hundley, H.A. et al. (2005) Science 308, 1032-4.
  3. Otto, H. et al. (2005) Proc Natl Acad Sci U S A 102, 10064-9.
  4. Richly, H. et al. (2010) Nature 468, 1124-8.
  5. Greiner, J. et al. (2003) Int J Cancer 106, 224-31.
  6. Resto, V.A. et al. (2000) Cancer Res 60, 5529-35.
  7. Tabarkiewicz, J. and Giannopoulos, K. (2010) Transplant Proc 42, 3293-6.
  8. Barilari, M. et al. (2017) EMBO J 36, 736-750.

Application References

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