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Product Pathways - Innate Immunity

AIM2 (D5X7K) Rabbit mAb #12948

Item# Description List Price Web Price Qty
12948S AIM2 (D5X7K) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 40 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Homology

Species predicted to react based on 100% sequence homology: Monkey.

Specificity / Sensitivity

AIM2 (D5X7K) Rabbit mAb recognizes endogenous levels of total AIM2 protein. An unknown band is detected at 22 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys93 of human AIM2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi, KARPAS-299, and L-540 cell lines using AIM2 (D5X7K) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HL-60 cells, serum-starved and either untreated (-) or treated overnight with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml; +), using AIM2 (D5X7K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-DDK tagged human AIM2 (hAIM2-Myc/DDK; +), using AIM2 (D5X7K) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).


IP

IP

Immunoprecipitation of AIM2 from HL-60 cell extracts treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, overnight) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AIM2 (D5X7K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using AIM2 (D5X7K) Rabbit mAb.

Background

Absent in melanoma 2 (AIM2) is an interferon-inducible protein containing an amino-terminal pyrin domain and carboxy-terminal HIN-200 domain that functions in innate immunity and tumor progression (1). Expression of AIM2 can inhibit cell growth and tumor formation (2,3). Furthermore, the AIM2 gene has a high frequency of mutations associated with microsatellite-unstable colorectal cancers (4). AIM2 has a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. Caspase-1 activation is regulated by multi-protein complexes referred to as “inflammasomes” (5,6). Distinct inflammasome complexes have been described containing NLRP1/NALP1, NLRP3/NALP3, IPAF, and AIM2. The HIN-200 domain of AIM2 is responsible for binding to cytoplasmic double stranded DNA, resulting in caspase-1 activation. (7-9). This inflammasome complex also involves binding of the pyrin domain of AIM2 to the CARD-domain protein ASC/TMS1, which then interacts directly with caspase-1. As a result, AIM2 has been demonstrated to be an important sensor for a number of different pathogens (10-12).

  1. DeYoung, K.L. et al. (1997) Oncogene 15, 453-7.
  2. Chen, I.F. et al. (2006) Mol Cancer Ther 5, 1-7.
  3. Patsos, G. et al. (2010) Int J Cancer 126, 1838-49.
  4. Woerner, S.M. et al. (2007) Genes Chromosomes Cancer 46, 1080-9.
  5. Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.
  6. Khare, S. et al. (2010) Crit Rev Immunol 30, 463-87.
  7. Roberts, T.L. et al. (2009) Science 323, 1057-60.
  8. Hornung, V. et al. (2009) Nature 458, 514-8.
  9. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13.
  10. Jones, J.W. et al. (2010) Proc Natl Acad Sci USA 107, 9771-6.
  11. Fernandes-Alnemri, T. et al. (2010) Nat Immunol 11, 385-93.
  12. Kim, S. et al. (2010) Eur J Immunol 40, 1545-51.

Application References

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