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XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

Syk (D3Z1E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #13714

Item# Description List Price Web Price Qty
13714S Syk (D3Z1E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) - 100 µl $498.00
$448.20
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
12081S Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate) - 100 µl $468.00
$421.20
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Application Dilution Species-Reactivity Sensitivity Isotype
F Human, Mouse, Rat Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: F=Flow Cytometry

Protocols

Specificity / Sensitivity

Syk (D3Z1E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total Syk protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Asn463 of human Syk protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of RL-7 cells using Syk (D3Z1E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (dashed line).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Syk (D3Z1E) XP® Rabbit mAb #13198.

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).

  1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
  2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
  3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
  4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
  5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
  6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
  7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
  8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
  9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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