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Product Pathways - Angiogenesis

ETS-1 (D8O8A) Rabbit mAb #14069

Item# Description List Price Web Price Qty
14069S ETS-1 (D8O8A) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat Endogenous 52 Rabbit IgG
IP
IF-IC
ChIP
ChIP-seq

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq

Directions For Use

For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Specificity / Sensitivity

ETS-1 (D8O8A) Rabbit mAb recognizes endogenous levels of total ETS-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ETS-1 (D8O8A) Rabbit mAb.

IP

IP

Immunoprecipitation of ETS-1 protein from OVCAR8 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ETS-1 (D8O8A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ETS-1 (D8O8A) Rabbit mAb

IF-IC

IF-IC

Confocal immunofluorescent analysis of OVCAR8 (high expression, left), A549 (moderate expression, center), and HeLa (low expression, right) cells using ETS-1 (D8O8A) Rabbit mAb (green). Actin filaments were labeled with Dylight™ 554 Phalloidin #13054 (red).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either ETS-1 (D8O8A) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human c-Myc intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and ETS-1 (D8O8A) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across RNF187 gene. For additional ChIP-seq tracks, please download the product data sheet.

Background

ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9).

  1. Bartel, F.O. et al. (2000) Oncogene 19, 6443-54.
  2. Sato, Y. (2001) Cell Struct Funct 26, 19-24.
  3. Oettgen, P. (2006) Circ Res 99, 1159-66.
  4. Dube, A. et al. (1999) Circ Res 84, 1177-85.
  5. Lelièvre, E. et al. (2000) Oncogene 19, 2438-46.
  6. Ghosh, S. et al. (2012) J Biol Chem 287, 15001-15.
  7. Verschoor, M.L. et al. (2010) PLoS One 5, e13565.
  8. Pognonec, P. et al. (1988) EMBO J 7, 977-83.
  9. Yang, B.S. et al. (1996) Mol Cell Biol 16, 538-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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