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SignalStain® Citrate Unmasking Solution (10X) #14746

Item# Description List Price Web Price Qty
14746P SignalStain® Citrate Unmasking Solution (10X) - 25 ml $35.00
$31.50
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14746S SignalStain® Citrate Unmasking Solution (10X) - 125 ml $130.00
$117.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Description

SignalStain® Citrate Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if citrate is recommended for your specific product.

Directions for Use

Dilute to a 1X unmasking solution using ddH2O just before use. Bring slides to a boil in 1X SignalStain® Citrate Unmasking Solution. Maintain at a sub-boiling temperature for 10 min. Cool slides on bench top for 30 min.

Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.

Background

Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues antigens (1).

  1. Yamashita, S. (2007) Prog Histochem Cytochem 41, 141-200.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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