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XP Monoclonal Antibody

Product Pathways - Lineage Markers

FAM3C (D1S2D) XP® Rabbit mAb #15171

Item# Description List Price Web Price Qty
15171S FAM3C (D1S2D) XP® Rabbit mAb - 100 µl $433.00
$389.70
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 25 Rabbit IgG
IHC-P
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

FAM3C (D1S2D) XP® Rabbit mAb recognizes endogenous levels of total FAM3C protein. Based on the sequence of the immunogenic peptide, the antibody is not expected to cross-react with other FAM3 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly169 of human FAM3C protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FAM3C (D1S2D) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FAM3C (D1S2D) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using FAM3C (D1S2D) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using FAM3C (D1S2D) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCT 116 cell pellet (left, high-expressing) or HeLa cell pellet (right, low-expressing) using FAM3C (D1S2D) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using FAM3C (D1S2D) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 cells (left, high-expressing) and HeLa cells (right, low-expressing) using FAM3C (D1S2D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and HCT 116 cells (green) using FAM3C (D1S2D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Background

FAM3C, also known as ILEI (interleukin-like epithelial-to-mesenchymal transition [EMT] inducer), is a cytokine-like protein and member of the FAM3 family. FAM3C plays an important role in EMT and metastasis during cancer progression in human and mouse cells, and is highly expressed in human cancer (1,2). In colorectal cancer, researchers have indicated that FAM3C is a marker for EMT and tumor progression, and that high expression of FAM3C is predictive of poor prognosis (3). While EMT induction by FAM3C can be independent of TGF-beta, research studies have also shown TGF-beta-dependent regulation of FAM3C expression at the translational level in mouse and human cells (4,5).

FAM3C has also been linked to regulation of osteoblast differentiation (6), and to accumulation of amyloid beta plaques in Alzheimer’s disease (7). FAM3C exists in monomeric and in homodimeric form, and research shows that FAM3C homodimers contain its EMT-inducing and tumor promoting activity (8).

  1. Waerner, T. et al. (2006) Cancer Cell 10, 227-39.
  2. Lahsnig, C. et al. (2009) Oncogene 28, 638-50.
  3. Gao, Z.H. et al. (2014) Histopathology 65, 527-38.
  4. Chaudhury, A. et al. (2010) Nat Cell Biol 12, 286-93.
  5. Song, Q. et al. (2014) Tumour Biol 35, 1377-82.
  6. Bendre, A. et al. Differentiation 93, 50-57.
  7. Liu, L. et al. (2016) Neuroscience 330, 236-46.
  8. Kral, M. et al. (2017) FEBS J 284, 3484-3505.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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