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Product Pathways - Tyrosine Kinase / Adaptors

Phospho-EGF Receptor (Tyr845) Antibody #2231

Item# Description List Price Web Price Qty
2231L Phospho-EGF Receptor (Tyr845) Antibody - 300 µl $1,004.00
$903.60
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2231S Phospho-EGF Receptor (Tyr845) Antibody - 100 µl $424.00
$381.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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2235S Phospho-EGF Receptor (Tyr992) Antibody - 100 µl $424.00
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2237S Phospho-EGF Receptor (Tyr1045) Antibody - 100 µl $424.00
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2237T Phospho-EGF Receptor (Tyr1045) Antibody - 20 µl $171.00
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4404S Phospho-EGF Receptor (Tyr1148) Antibody - 100 µl $424.00
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4407L Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb - 300 µl $1,007.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $106.00
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9922T Phospho-EGF Receptor Antibody Sampler Kit - 1 Kit $444.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat Endogenous 175 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

Phospho-EGF Receptor (Tyr845) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 845. The antibody may cross-react with other activated EGF receptor family members (e.g., ErbB2).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr845 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr845) Antibody (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower).

Background

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

  1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
  2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
  3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
  4. Hubbard, S.R. et al. (1994) Nature 372, 746-54.
  5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
  6. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
  7. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
  8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
  9. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
  10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.

Application References

  • Wu, W. et al. (2002) J. Biol. Chem. 277, 24252-24257. Applications: Western Blotting.
  • Iordanov, M. S. et al. (2002) Mol. Cell. Biol. 22, 5380-5394. Applications: Western Blotting.
  • Zhang, B. et al. (2002) J Virol 76, 220-31. Applications: Western Blotting.
  • Ravid, T. et al. (2002) J Biol Chem 277, 31214-9. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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