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Product Pathways - Angiogenesis

FGF Receptor 2 (D4L2V) Rabbit mAb #23328

Item# Description List Price Web Price Qty
23328S FGF Receptor 2 (D4L2V) Rabbit mAb - 100 µl $377.00
$339.30
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse Endogenous 92,145 Rabbit IgG
IP
IHC-P
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

FGF Receptor 2 (D4L2V) Rabbit mAb recognizes endogenous levels of total FGFR2 protein and does not cross-react with FGFR1 or FGFR4.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FGFR2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FGF Receptor 2 (D4L2V) Rabbit mAb.

IP

IP

Immunoprecipitation of FGFR2 protein from Saos-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FGF Receptor 2 (D4L2V) Rabbit mAb. Western blot analysis was performed using FGF Receptor 2 (D4L2V) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 2 (D4L2V) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FGF Receptor 2 (D4L2V) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 2 (D4L2V) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded KATO III (high expressing; left), AN3 CA (low expressing; center), and SW620 (negative; right) cell pellets using FGF Receptor 2 (D4L2V) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of KATO III (high expressing; left), AN3 CA (low expressing; center), and SW620 (negative; right) cells using FGF Receptor 2 (D4L2V) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SW620 (blue) and KATO III (green) cells using FGF Receptor 2 (D4L2V) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

FGFR2 has several splicing isoforms, with ligand specificity largely determined by alternative splicing of exons 8 (IIIb) and 9 (IIIc). Alternative splicing is cell type specific, resulting in isoforms showing various tissue distribution and biological activities (6,7). Research studies have shown that mutations in the corresponding FGFR2 gene cause syndromes characterized by facial and limb defects, including LADD Syndrome, Crouzon Syndrome, Beare-Stevenson Cutis Gyrata Syndrome, Pfeiffer Syndrome, Apert Syndrome, and Jackson-Weiss Syndrome (8-10). Investigators have also observed mutations and altered expression of FGFR2 in cases of gastric, endometrial, and breast cancer (11).

  1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
  2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
  3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
  4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
  5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.
  6. Muh, S.J. et al. (2002) J Biol Chem 277, 50143-54.
  7. Coutts, J.C. and Gallagher, J.T. (1995) Immunol Cell Biol 73, 584-9.
  8. Jeftha, A. et al. (2004) J Clin Pediatr Dent 28, 173-6.
  9. Wilkinson, C.C. et al. (2012) Childs Nerv Syst 28, 1221-6.
  10. Slavotinek, A. et al. (2009) Am J Med Genet A 149A, 1814-7.
  11. Katoh, M. (2009) J Invest Dermatol 129, 1861-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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