New England Biolabs Canada
 

Tag Antibodies

GST (26H1) Mouse mAb #2624

Item# Description List Price Web Price Qty
2624S GST (26H1) Mouse mAb - 100 µl $372.00
$334.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Sensitivity Isotype
W Transfected Only Mouse IgG2a
IP
IF-IC

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

GST (26H1) Mouse mAb detects transfected glutathione S-transferase (GST) fusion proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from transfected COS-7 cells overexpressing GST-Bad and untransfected control, using GST (26H1) Mouse mAb (left) or anti-Bad Antibody (right).

IF-IC

IF-IC

Immunocytochemical staining of COS-7 cells expressing GST-PAK2 protein (left) or cells without transfected protein (right), using GST (26H1) Mouse mAb.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

  1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
  2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
  3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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