New England Biolabs Canada
 

Product Pathways - Neuroscience

DYRK1B Antibody #2703

Item# Description List Price Web Price Qty
2703S DYRK1B Antibody - 100 µl $372.00
$334.80
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey Endogenous 70-80 Rabbit
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

DYRK1B Antibody detects endogenous levels of total DYRK1B protein. This antibody detects the three major alternative splicing variants reported for DYRK1B.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human DYRK1B. Antibodies were purified by protein affintiy chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and rat testes using DYRK1B Antibody.

Background

The DYRK family includes several dual-specificity tyrosine-phosphorylated and regulated kinases capable of phosphorylating proteins at both Tyr and Ser/Thr residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the Drosophila minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop; phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) is necessary for kinase activity. DYRKs typically autophosphorylate the Tyr residue within their activation loop, but phosphorylate substrates at Ser and Thr residues (1,6).

In contrast to the ubiquitous DYRK1A, DYRK1B exhibits relatively restricted expression with highest levels found in the testis and muscle (7,8). Three major DYRK1B splice variants demonstrate distinct expression patterns and functional properties (9). DYRK1B plays a critical role in myoblast differentiation by affecting cell motility, transcription, cell cycle progression and survival (10,11). In addition, DYRK1B contributes to the survival of certain cancer cells (7,12,13).

  1. Becker, W. and Joost, H.G. (1999) Prog. Nucleic Acid Res. Mol. Biol. 62, 1-17.
  2. Garrett, S. and Broach, J. (1989) Genes Dev. 3, 1336-1348.
  3. Tejedor, F. et al. (1995) Neuron 14, 287-301.
  4. Kentrup, H. et al. (1996) J. Biol. Chem. 271, 3488-3495.
  5. Becker, W. et al. (1998) J. Biol. Chem. 273, 25893-25902.
  6. Lochhead, P.A. et al. (2005) Cell 121, 925-936.
  7. Leder, S. et al. (1999) Biochem. Biophys. Res. Commun. 254, 474-479.
  8. Lee, K. et al. (2000) Cancer Res. 60, 3631-3637.
  9. Leder, S. et al. (2003) Biochem. J. 372, 881-888.
  10. Mercer, S.E. and Friedman, E. (2006) Cell Biochem. Biophys. 45, 303-315.
  11. Deng, X. et al. (2003) J. Biol. Chem. 278, 41347-41354.
  12. Deng, X. et al. (2006) Cancer Res. 66, 4149-4158.
  13. Mercer, S.E. et al. (2006) Cancer Res. 66, 5143-5150.

Application References

  • Litovchick, L. et al. (2011) Genes Dev 25, 801-13. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites