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Normal Rabbit IgG #2729

Item# Description List Price Web Price Qty
2729S Normal Rabbit IgG - 250 µl $113.00
$101.70
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Applications Concentration Sensitivity Isotype
Immunoprecipitation
Chromatin IP
1 mg/ml Endogenous Rabbit

Directions For Use

Important! Dilute this control antibody to the same concentration (not dilution) as the specific test antibody in the chromatin immunoprecipitation. This is typically 1 μl (1 μg) to 5 μl (5 μg) of control antibody for one immunoprecipitation. Higher background signal may result if an excessive amount of rabbit IgG isotype control is used.

Source / Purification

The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.

IP

IP

Immunoprecipitation of 4E-BP2 from C2C12 cell extracts using Normal Rabbit IgG #2729 (lane 2) or 4E-BP2 Antibody #2845 (lane 3). Lane 1 is 10% input. Western blot analysis was performed using 4E-BP2 Antibody #2845.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and either Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620 (lane 2), Rpb1 CTD (4H8) Mouse mAb #2629 (lane 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (lane 4) or Normal Rabbit IgG #2729 (lane 5). Purified DNA was analyzed by standard PCR methods using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human a Satellite Repeat Primers #4486. PCR products were observed for each primer set in the input sample (lane 1) and various protein-specific immunoprecipitations but no PCR products were observed with immunoprecipitation using Normal Rabbit IgG #2729 (lane 5).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The relative abundance of each DNA sequence enriched by Normal Rabbit IgG (red) is compared to the amount of the same DNA sequence enriched by the protein-specific immunoprecipitations.


Description

Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific IgG control in chromatin immunoprecipitation using our SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003.

Background

Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Application References

  • Kong, D.K. et al. (2010) Mol Biol Cell 21, 1335-49. Applications: Chromatin IP.
  • Shukla, S. et al. (2011) Nature 479, 74-9. Applications: Chromatin IP.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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