New England Biolabs Canada
 

Product Pathways - Protein Stability

CUL3 Antibody #2759

Item# Description List Price Web Price Qty
2759S CUL3 Antibody - 100 µl $372.00
$334.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey Endogenous 82, 84 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

CUL3 Antibody detects endogenous levels of total Cullin-3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu750 of human Cullin-3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lysates, using CUL3 Antibody.

Background

CUL3 (Cullin-3) is a member of the cullin-based ubiquitin ligase family. By interacting with Hrt1 and BTB domain containing proteins, the complex functions as a CUL3-based E3 ligase to bring specific substrates to ubiquitinylation and degradation (1). The CUL3 complex has been shown to target many substrates involved in cell cycle progression (2), transcription (3), development and differentiation (4,5).

  1. Pintard, L. et al. (2004) EMBO J. 23, 1681-1687.
  2. Singer, J.D. et al. (1999) Genes Dev. 13, 2375-2387.
  3. Furukawa, M. and Xiong, Y. (2005) Mol Cell Biol 25, 162-171.
  4. Zhu, S. et al. (2005) J. Neurosci. 25, 4189-4197.
  5. Ou, C.Y. et al. (2002) Genes Dev. 16, 2403-2414.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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