New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - CD Markers

F4/80 (D4C8V) XP® Rabbit mAb #30325

Item# Description List Price Web Price Qty
30325S F4/80 (D4C8V) XP® Rabbit mAb - 100 µl $419.00
$377.10
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30325T F4/80 (D4C8V) XP® Rabbit mAb - 20 µl $174.00
$156.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Mouse Endogenous 65-250 Rabbit IgG
IP
IF-F
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

F4/80 (D4C8V) XP® Rabbit mAb recognizes endogenous levels of total F4/80 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln260 of mouse F4/80 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using F4/80 (D4C8V) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of F4/80 from RAW 264.7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is F4/80 (D4C8V) XP® Rabbit mAb. Western blot analysis was performed using F4/80 (D4C8V) XP® Rabbit mAb as the primary antibody and Mouse Anti-Rabbit IgG (Conformation Specific) (L29A9) mAb #3678, followed by Anti-mouse IgG, HRP-linked Antibody #7076 as the secondary antibodies.

IF-F

IF-F

Confocal immunofluorescent analysis of Tg2576 brain, which overexpresses mutant human APP695. Sections were labeled with F4/80 (D4C8V) XP® Rabbit mAb (green), β-Amyloid (D3D2N) Mouse mAb #15126 (yellow), and GFAP (E6N9L) Mouse mAb #34001 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


IF-F

IF-F

Confocal immunofluorescent analysis of normal mouse spleen using F4/80 (D4C8V) XP® Rabbit mAb (red) and B220/CD45R Alexa Fluor® 488 conjugate (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

IF-IC

IF-IC

Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) or Neuro-2a cells (right, negative), using F4/80 (D4C8V) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Background

F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4).

  1. Hirsch, S. et al. (1981) J Exp Med 154, 713-25.
  2. Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
  3. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  4. Greter, M. et al. (2015) Front Immunol 6, 249.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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