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β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb #32098

Item# Description List Price Web Price Qty
32098S β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb - 100 µl $364.00
$327.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse Endogenous 6 Rabbit IgG
IF-F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen)

Protocols

Specificity / Sensitivity

β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb recognizes endogenous levels of total human Aβ-43 protein. This product detects transgenically expressed human APP in mouse models. This antibody weakly cross-reacts with human Aβ-42 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy of human β-Amyloid (1-43) protein.

Western Blotting

Western Blotting

Western blot analysis with the indicated amounts of human β-amyloid (1-43) protein using β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42 and Aβ-43 peptides (10 ng) using β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb. Note the slight cross-reactivity with Aβ-42.

IF-F

IF-F

Confocal immunofluorescent analysis of brain from the 5XFAD mouse model of Alzheimer's disease using β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red pseudocolor) and APP (D54D2) Rabbit mAb (Alexa Fluor® 647 Conjugate) #42284 (yellow pseudocolor). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

Aβ43 has been suggested as a biomarker in early onset of Alzheimer's disease, where patients have lower levels of Aβ43 in cerebrospinal fluid (7,8,9). Several studies have shown that Aβ toxicity of Aβ43 is as high as Aβ42 or Aβ40 in different models of Alzheimer's disease, including mouse models and human disease. (10).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
  3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
  4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
  5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
  6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.
  7. Lauridsen, C. et al. (2017) Front Aging Neurosci 9, 210.
  8. Almdahl, I.S. et al. (2017) Front Aging Neurosci 9, 9.
  9. Pachahara, S.K. et al. (2015) PLoS One 10, e0136567.
  10. Trambauer, J. et al. (2017) Methods Enzymol 584, 157-183.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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