New England Biolabs Canada
 

Product Pathways - Neuroscience

GFAP (E6N9L) Mouse mAb #34001

Item# Description List Price Web Price Qty
34001S GFAP (E6N9L) Mouse mAb - 100 µl $364.00
$327.60
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
12389S GFAP (D1F4Q) XP® Rabbit mAb - 100 µl $410.00
$369.00
ADD TO CART
12389T GFAP (D1F4Q) XP® Rabbit mAb - 20 µl $171.00
$153.90
ADD TO CART
12630P SignalFire™ Plus ECL Reagent - 20 ml $113.00
$101.70
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $508.00
$457.20
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $120.00
$108.00
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $546.00
$491.40
ADD TO CART
12957S Western Blotting Application Solutions Kit - 1 Kit $587.00
$528.30
ADD TO CART
13953L Prestained Protein Marker, Broad Range (11-190 kDa) - 1750 µl $486.00
$437.40
ADD TO CART
13953P Prestained Protein Marker, Broad Range (11-190 kDa) - 50 µl $59.00
$53.10
ADD TO CART
13953S Prestained Protein Marker, Broad Range (11-190 kDa) - 350 µl $119.00
$107.10
ADD TO CART
3670S GFAP (GA5) Mouse mAb - 100 µl $364.00
$327.60
ADD TO CART
3670T GFAP (GA5) Mouse mAb - 20 µl $155.00
$139.50
ADD TO CART
37478P Protein G Agarose Beads - 200 µl $97.00
$87.30
ADD TO CART
37478S Protein G Agarose Beads - 1 ml $196.00
$176.40
ADD TO CART
61656S Mouse (E5Y6Q) mAb IgG2a Isotype Control - 100 µl $161.00
$144.90
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $42.00
$37.80
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $363.00
$326.70
ADD TO CART
7003P 20X LumiGLO® Reagent and 20X Peroxide - 5 ml each $90.00
$81.00
ADD TO CART
7003S 20X LumiGLO® Reagent and 20X Peroxide - 25 ml each $287.00
$258.30
ADD TO CART
7071S Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody - 50 assays $395.00
$355.50
ADD TO CART
7072S Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody - 50 assays $395.00
$355.50
ADD TO CART
7075S Anti-biotin, HRP-linked Antibody - 1 ml $189.00
$170.10
$141.75
ADD TO CART
7076P2 Anti-mouse IgG, HRP-linked Antibody - 100 µl $106.00
$95.40
$79.50
ADD TO CART
7076S Anti-mouse IgG, HRP-linked Antibody - 1 ml $199.00
$179.10
$149.25
ADD TO CART
7076V Anti-mouse IgG, HRP-linked Antibody - 5 ml $633.00
$569.70
$474.75
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $319.00
$287.10
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $90.00
$81.00
ADD TO CART
9774T Loading Control Antibody Sampler Kit (Mouse) - 1 Kit $354.00
$318.60
ADD TO CART
9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $88.00
$79.20
ADD TO CART
9999S Nonfat Dry Milk - 250 g $84.00
$75.60
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat Endogenous 50 Mouse IgG2a
IP
IF-F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen)

Specificity / Sensitivity

GFAP (E6N9L) Mouse mAb recognizes endogenous levels of total GFAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues and cell lines using GFAP (E6N9L) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain using GFAP (E6N9L) Mouse mAb as the primary antibody. Various anti-mouse isotype specific antibodies (upper) and Anti-mouse IgG, HRP-linked Antibody #7076 (lower) were used as secondary antibodies.

IP

IP

Immunoprecipitation of GFAP protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656, and lane 3 is GFAP (E6N9L) Mouse mAb. Western blot analysis was performed GFAP (E6N9L) Mouse mAb.


IF-F

IF-F

Confocal immunofluorescent analysis of mouse Tg2576 brain, which overexpresses mutant human APP695. Sections were labeled with GFAP (E6N9L) Mouse mAb (green, mouse IgG2a), β-Amyloid (D3D2N) Mouse mAb #15126 (red, mouse IgG1), and HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) #3892 (magenta, rabbit IgG). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).

  1. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
  3. Jessen, K.R. et al. (1990) Development 109, 91-103.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites