New England Biolabs Canada
 

Product Pathways - Cell Cycle / Checkpoint

Chk2 (1C12) Mouse mAb #3440

Item# Description List Price Web Price Qty
3440S Chk2 (1C12) Mouse mAb - 100 µl $372.00
$334.80
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3440T Chk2 (1C12) Mouse mAb - 20 µl $159.00
$143.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Monkey Endogenous 62 Mouse IgG2b
IHC-P
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

Chk2 (1C12) Mouse mAb detects endogenous levels of total Chk2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with truncated recombinant GST-Chk2.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Chk2 (1C12) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Chk2 (1C12) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Chk2 (1C12) Mouse mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Chk2 (1C12) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

Application References

  • Bartucci, M. et al. (2011) Cell Death Differ , . Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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