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Product Pathways - Translational Research

CMTM6 Antibody #34557

Item# Description List Price Web Price Qty
34557S CMTM6 Antibody - 100 µl $364.00
$327.60
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse Endogenous 22 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

CMTM6 Antibody recognizes endogenous levels of total CMTM6 protein. This antibody does not cross-react with CMTM4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human CMTM6 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CMTM6 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human CMTM6 (CMTM6-Myc/DDK), Myc/DDK-tagged full-length mouse CMTM6 (CMTM6 mouse-Myc/DDK), Myc/DDK-tagged full-length human CMTM4 (CMTM4-Myc/DDK), Myc/DDK-tagged full-length mouse CMTM4 (CMTM4 mouse-Myc/DDK), using CMTM6 Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-actin (D6A8) Rabbit mAb #8457 (lower).

Background

CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6) is a member of the chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (1). CMTM6 stabilizes plasma membrane expression of PD-L1, an immune inhibitory ligand critical for immune tolerance to self and anti-tumor immunity (2,3). CMTM6 associates with PD-L1 at recycling endosomes, where it protects PD-L1 from being targeted for lysosomal degradation by preventing STUB1-mediated PD-L1 ubiquitination (2,3). CMTM6 may stabilize PD-L1 expression on antigen presenting cells and potentiate inhibitory signaling by PD-1 on T cells, triggering T cell inhibition and exhaustion. CMTM6 has also been shown to interact with with CD58, ARG1, ENO1, and TMPO (2). Due to the role of CMTM6 in regulating the immune system, it is being investigated as an immunotherapeutic target for the treatment of cancer.

  1. Han, W. et al. (2003) Genomics 81, 609-17.
  2. Burr, M.L. et al. (2017) Nature 549, 101-5.
  3. Mezzadra, R. et al. (2017) Nature 549, 106-10.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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