New England Biolabs Canada
 

Product Pathways - Lymphocyte Signaling

4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb #34594

Item# Description List Price Web Price Qty
34594S 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb - 100 µl $385.00
$346.50
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 32, 40 Rabbit IgG
IP
IHC-P
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb recognizes endogenous levels of total 4-1BB/CD137/TNFRSF9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the extracellular domain of human 4-1BB/CD137/TNFRSF9 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from primary human CD8+ T cells, treated with anti-CD3 and anti-CD28 in the presence of human interleukin-2, and various cell lines using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of 4-1BB/CD137/TNFRSF9 from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb. Western blot analysis was performed using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HDLM-2 cell pellet (left, positive) or HT-29 cell pellet (right, negative) using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human thymus using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of primary human CD8+ T cells treated with anti-CD3 and anti-CD28 in the presence of human interleukin-2 (left, positive) and HeLa cells (right, negative) using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells (green), treated with anti-CD3 (10 μg/ml, 72h) and anti-CD28 (5 μg/ml, 72h), using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed lines) co-stained with a CD8 antibody. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on CD8+ cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and HDLM-2 cells (green), using 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Background

TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1, 2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6).

  1. Sun, Y. et al. (2002) Nat Med 8, 1405-13.
  2. Wilcox, R.A. et al. (2002) J Clin Invest 109, 651-9.
  3. Lin, W. et al. (2008) Blood 112, 699-707.
  4. Wilcox, R.A. et al. (2002) J Immunol 168, 4262-7.
  5. Melero, I. et al. (1997) Nat Med 3, 682-5.
  6. Yonezawa, A. et al. (2015) Clin Cancer Res 21, 3113-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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