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GAPDH (14C10) Rabbit mAb (HRP Conjugate) #3683

Item# Description List Price Web Price Qty
3683S GAPDH (14C10) Rabbit mAb (HRP Conjugate) - 100 µl $436.00
$392.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey, Bovine, Pig Endogenous 37 Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: W=Western Blotting

Homology

Species predicted to react based on 100% sequence homology: Pig.

Protocols

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb (HRP Conjugate) detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. The antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, HeLa and C6 cells using GAPDH (14C10) Rabbit mAb (HRP Conjugate).

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The unconjugated GAPDH (14C10) Rabbit mAb #2118 reacts with human, mouse, rat and monkey GAPDH. CST expects that GAPDH (14C10) (HRP conjugate) #3683 will also recognize GAPDH in these species.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

  1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
  2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
  3. Zheng, L. et al. (2003) Cell 114, 255-66.
  4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
  5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

Application References

  • Han, W. et al. (2011) PLoS One 6, e18691. Applications: Western Blotting.
  • Muller, F.L. et al. (2012) Nature 488, 337-42. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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