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Product Pathways - Lymphocyte Signaling

MAGE-A3 (E9S4X) Rabbit mAb #38896

Item# Description List Price Web Price Qty
38896S MAGE-A3 (E9S4X) Rabbit mAb - 100 µl $364.00
$327.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 45 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

MAGE-A3 (E9S4X) Rabbit mAb recognizes endogenous levels of total MAGE-A3 protein. This antibody cross-reacts with MAGE-A2 and MAGE-A12 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAGE-A3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MAGE-A3 (E9S4X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MAGE-A3 protein is not detected in HCC1143 cells.

Western Blotting

Western Blotting

Western blot anlysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human MAGE-A2 (hMAGE-A2-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A3 (hMAGE-A3-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A4 (hMAGE-A4-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A6 (hMAGE-A6-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A10 (hMAGE-A10-Myc/DDK), and Myc/DDK-tagged full-length human MAGE-A12 (hMAGE-A12-Myc/DDK), using MAGE-A3 (E9S4X) Rabbit mAb (upper), DYKDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of MAGE-A3 from SK-MEL-28 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MAGE-A3 (E9S4X) Rabbit mAb. Western blot analysis was performed using MAGE-A3 (E9S4X) Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.


Background

Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3).

Melanoma antigen-A3 (MAGE-A3) is a cancer testis antigen and belongs to the type I MAGE family of proteins. The expression of MAGE-A3 is normally restricted to the human testis but is aberrantly upregulated in a number of human cancers, such as lung cancer, colorectal cancer, and multiple myeloma (4-6). Research studies have recently demonstrated that MAGE-A3 drives tumorigenesis as part of the MAGE-A3-TRIM28 ubiquitin ligase complex that promotes proteasomal degradation of the tumor suppressor kinase AMPK (7). Due to its upregulated and selective expression in human tumors and high degree of immunogenicity, MAGE-A3 has received significant attention as a novel immunotherapy target through the use of vaccines and adoptive cell therapy (8,9).

  1. Caballero, O.L. and Chen, Y.T. (2009) Cancer Sci 100, 2014-21.
  2. De Smet, C. et al. (1999) Mol Cell Biol 19, 7327-35.
  3. Gjerstorff, M.F. et al. (2015) Oncotarget 6, 15772-87.
  4. Jang, S.J. et al. (2001) Cancer Res 61, 7959-63.
  5. Shantha Kumara, H.M. et al. (2012) Cancer Immun 12, 16.
  6. Atanackovic, D. et al. (2007) Blood 109, 1103-12.
  7. Pineda, C.T. et al. (2015) Cell 160, 715-28.
  8. Straetemans, T. et al. (2012) Clin Dev Immunol 2012, 586314.
  9. Esfandiary, A. and Ghafouri-Fard, S. (2015) Immunotherapy 7, 683-704.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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