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XP Monoclonal Antibody

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Rabbit (DA1E) mAb IgG XP® Isotype Control #3900

Item# Description List Price Web Price Qty
3900S Rabbit (DA1E) mAb IgG XP® Isotype Control - 250 µg $184.00
$165.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Applications Concentration Sensitivity Isotype
Immunoprecipitation
Immunohistochemistry (Paraffin)
Immunofluorescence (Immunocytochemistry)
Flow Cytometry
Chromatin IP
2.5 mg/ml Endogenous Rabbit IgG

Directions For Use

Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background may result if excessive amounts of rabbit IgG isotype control is used.

Specificity / Sensitivity

Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells using β-Tubulin (9F3) Rabbit mAb #2128 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells, untreated (green) or STI-treated (blue), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The relative abundance of each DNA sequence enriched by Rabbit (DA1E) mAb IgG XP® Isotype Control (red) is compared to the amount of the same DNA sequence enriched by the histone H3-specific immunoprecipitations (blue).

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Application References

  • Zhang, S.J. et al. (2011) Chin Med J (Engl) 124, 3118-26. Applications: Flow Cytometry.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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