New England Biolabs Canada
 

Product Pathways - Angiogenesis

MMP-2 Antibody #4022

Item# Description List Price Web Price Qty
4022S MMP-2 Antibody - 100 µl $364.00
$327.60
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human Endogenous 64, 72 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat.

Protocols

Specificity / Sensitivity

MMP-2 Antibody detects full length (proenzyme, 72 kDa) and cleaved (active enzyme, 64 kDa) MMP-2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys116 of human MMP-2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-2, using MMP-2 Antibody.

Background

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

  1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
  2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
  3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
  4. Vu, T.H. et al. (1998) Cell 93, 411-22.
  5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites