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DRAQ5® #4084

Item# Description List Price Web Price Qty
4084L DRAQ5® - 200 µl $615.00
$553.50
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4084S DRAQ5® - 50 µl $297.00
$267.30
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
4753T Cellular Localization IF Antibody Sampler Kit - 1 Kit $845.00
$760.50
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Background

DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).

  1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
  2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
  3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (PE Conjugate) #29237 versus DRAQ5® (DNA content).

Spectra

Spectra

Absorption and fluorescence emission spectra of DRAQ5® in aqueous solution.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).


Directions For Use

Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:

Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.

Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

Application References

  • Galitovskiy, V. et al. (2011) J Immunol 187, 2677-87. Applications: IF-IC (In Cells).
  • Talchai, C. et al. (2012) Cell 150, 1223-34. Applications: IF-IC (In Cells).
  • Talchai, C. et al. (2012) Nat Genet 44, 406-12, S1. Applications: IF-IC (In Cells).

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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