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Product Pathways - Jak/Stat Pathway

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb #4113

Item# Description List Price Web Price Qty
4113S Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb - 100 µl $423.00
$380.70
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human, Mouse, Rat, Monkey Endogenous 79, 86 Mouse IgG1
IP 1:100
IHC-P 1:100
IF-IC 1:100
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with IFN-α (100 ng/ml) for 5 minutes, using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (left) or Stat3 Antibody #9132 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical anlysis of paraffin-embedded human breast carcinoma using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb on SignalSlide® HeLa -/+ IFNa IHC Controls #55861 (paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (right)).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFN-alpha treated (right), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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