New England Biolabs Canada

Product Pathways - Jak/Stat Pathway

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb #4113

Item# Description List Price Web Price Qty
4113S Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb - 100 µl $391.00 $351.90 ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at

Companion Products

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1195S Phospho-Stat3 (Tyr705) Blocking Peptide - 100 µg $111.00 $99.90 ADD TO CART
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12757S SignalFire Elite ECL Reagent - 100 ml $503.00 $452.70 ADD TO CART
6883P3 SignalFire ECL Reagent - 50 ml $41.00 $36.90 ADD TO CART
6883S SignalFire ECL Reagent - 500 ml $335.00 $301.50 ADD TO CART
7072S Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody - 50 assays $363.00 $326.70 ADD TO CART
7076P2 Anti-mouse IgG, HRP-linked Antibody - 100 µl $97.00 $87.30 ADD TO CART
7076S Anti-mouse IgG, HRP-linked Antibody - 1 ml $181.00 $162.90 ADD TO CART
7076V Anti-mouse IgG, HRP-linked Antibody - 5 ml $573.00 $515.70 ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3,250 µl $293.00 $263.70 ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $83.00 $74.70 ADD TO CART
8105S SignalSlide® Phospho-Stat1/3/5 IHC Controls - 1 Pack $181.00 $162.90 ADD TO CART
8112L SignalStain® Antibody Diluent - 100 ml $111.00 $99.90 ADD TO CART
8112S SignalStain® Antibody Diluent - 25 ml $55.00 $49.50 ADD TO CART
9131L Phospho-Stat3 (Tyr705) Antibody - 300 µl $923.00 $830.70 ADD TO CART
9131S Phospho-Stat3 (Tyr705) Antibody - 100 µl $391.00 $351.90 ADD TO CART
9133S Stat3 Control Cell Extracts - 100 µl $153.00 $137.70 ADD TO CART
9134L Phospho-Stat3 (Ser727) Antibody - 300 µl $923.00 $830.70 ADD TO CART
9134P Phospho-Stat3 (Ser727) Antibody - 40 µl $195.00 $175.50 ADD TO CART
9134S Phospho-Stat3 (Ser727) Antibody - 100 µl $391.00 $351.90 ADD TO CART
9136S Phospho-Stat3 (Ser727) (6E4) Mouse mAb - 100 µl $391.00 $351.90 ADD TO CART
9138L Phospho-Stat3 (Tyr705) (3E2) Mouse mAb - 300 µl $923.00 $830.70 ADD TO CART
9138S Phospho-Stat3 (Tyr705) (3E2) Mouse mAb - 100 µl $391.00 $351.90 ADD TO CART
9139P Stat3 (124H6) Mouse mAb - 40 µl $181.00 $162.90 ADD TO CART
9139S Stat3 (124H6) Mouse mAb - 100 µl $335.00 $301.50 ADD TO CART
9145L Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb - 300 µl $1,021.00 $918.90 ADD TO CART
9145P Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb - 40 µl $209.00 $188.10 ADD TO CART
9145S Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb - 100 µl $433.00 $389.70 ADD TO CART
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human, Mouse, Rat, Monkey Endogenous 79, 86 Mouse IgG1
IP 1:100
IHC-P 1:50
IF-IC 1:100
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with IFN-α (100 ng/ml) for 5 minutes, using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (left) or Stat3 Antibody #9132 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical anlysis of paraffin-embedded human breast carcinoma using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right).



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFN-alpha treated (right), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.


The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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