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Product Pathways - Cell Cycle / Checkpoint

Cyclin B1 Antibody #4138

Item# Description List Price Web Price Qty
4138S Cyclin B1 Antibody - 100 µl $372.00
$334.80
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4138T Cyclin B1 Antibody - 20 µl $159.00
$143.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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13495S Phospho-Cyclin B1 (Ser116) Antibody - 100 µl $433.00
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4131S Phospho-Cyclin B1 (Ser147) Antibody - 100 µl $433.00
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4133S Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb - 100 µl $433.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
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9111L Phospho-cdc2 (Tyr15) Antibody - 300 µl $1,025.00
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9111S Phospho-cdc2 (Tyr15) Antibody - 100 µl $433.00
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9111T Phospho-cdc2 (Tyr15) Antibody - 20 µl $174.00
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9527S Phospho-cdc25C (Thr48) Antibody - 100 µl $433.00
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9527T Phospho-cdc25C (Thr48) Antibody - 20 µl $174.00
$99.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Hamster, Monkey Endogenous 55 Rabbit
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

Cyclin B1 Antibody detects endogenous levels of total cyclin B1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a syntheic peptide corresponding to residues near the amino terminus of human cyclin B1. Antibodies are purified using peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Cyclin B1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Cyclin B1 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) showing concentrated cyclin B1 staining surrounding mitotic chromatin.

Background

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).

  1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
  2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
  3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
  4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
  5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
  6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
  7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
  8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
  9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
  10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
  11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
  12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
  13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
  14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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