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Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) #4418

Item# Description List Price Web Price Qty
4418S Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) - 250 µl $173.00
$155.70
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Specificity / Sensitivity

IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells using HSP70 (6B3) Rat mAb #4873 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (green) and compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb #4736 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (blue) and COX IV (3E11) Rabbit mAb #4850 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Description

Anti-rat IgG (H+L) was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml.

Directions For Use

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.

Background

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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