New England Biolabs Canada
 

Product Pathways - Cell Cycle / Checkpoint

ORC1 (7A7) Rat mAb #4731

Item# Description List Price Web Price Qty
4731S ORC1 (7A7) Rat mAb - 100 µl $372.00
$334.80
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4731T ORC1 (7A7) Rat mAb - 20 µl $159.00
$143.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Hamster Endogenous 100, 95 (Mouse) Rat IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

ORC1 (7A7) Rat mAb recognizes endogenous levels of total ORC1 protein. The antibody does not cross-react with other ORC subunits.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a His6-tagged recombinant human ORC1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ORC1 (7A7) Rat mAb.

Background

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).

  1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
  2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
  3. Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
  4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
  5. Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
  6. DePamphilis, M.L. (2003) Gene 310, 1-15.
  7. McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.

Application References

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