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Product Pathways - NF-kB Signaling

Phospho-NF-κB2 p100 (Ser866/870) Antibody #4810

Item# Description List Price Web Price Qty
4810L Phospho-NF-KappaB2 p100 (Ser866/870) Antibody - 300 µl $1,057.00
$951.30
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4810S Phospho-NF-KappaB2 p100 (Ser866/870) Antibody - 100 µl $446.00
$401.40
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4810T Phospho-NF-κB2 p100 (Ser866/870) Antibody - 20 µl $180.00
$162.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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2697S Phospho-IKKAlpha/Beta (Ser176/180) (16A6) Rabbit mAb - 100 µl $446.00
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2697T Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb - 20 µl $180.00
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3017T NF-κB2 p100/p52 (18D10) Rabbit mAb - 20 µl $163.00
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3033T Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb - 20 µl $180.00
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4727S c-Rel Antibody - 100 µl $383.00
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4727T c-Rel Antibody - 20 µl $163.00
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4806S Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb - 100 µl $446.00
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4882S NF-KappaB2 p100/p52 Antibody - 100 µl $383.00
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4882T NF-κB2 p100/p52 Antibody - 20 µl $163.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse Transfected Only 110 Rabbit
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine, Dog.

Specificity / Sensitivity

Phospho-NF-κB2 p100 (Ser866/870) Antibody detects transfected NF-κB2 p100 when phosphorylated at serines 866 and 870.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serines 866/870 of NF-κB2 p100. Nomenclature refers to the human sequence (SwissProt# Q00653). This site is homologous to rat Ser864/868 (Q5U2Z4) and mouse Ser865/869 (Q9WTK5). Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with wild-type or mutant NF-κB2 p100 (SS866/870AA) and with or without NIK, using Phospho-NF-κB2 p100 (Ser866/870) Antibody or total NF-κB2 p100 Antibody #4882. The p100 constructs were generously provided by Dr. Warner Greene of the Gladstone Institute of Virology and Immunology, Dr. Shao-Cong Sun of The Pennsylvania State University College of Medicine, and Dr. Gutian Xiao of Rutgers, The State University of New Jersey.

Background

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

  1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
  3. Haskill, S. et al. (1991) Cell 65, 1281-9.
  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
  5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
  8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  9. Senftleben, U. et al. (2001) Science 293, 1495-9.
  10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
  11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Application References

  • Saitoh, Y. et al. (2008) Blood 111, 5118-29. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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