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Product Pathways - Cytoskeletal Signaling

COX IV (3E11) Rabbit mAb #4850

Item# Description List Price Web Price Qty
4850S COX IV (3E11) Rabbit mAb - 100 µl $372.00
$334.80
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4850T COX IV (3E11) Rabbit mAb - 20 µl $159.00
$143.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Rat, Monkey, Zebrafish, Bovine, Pig Endogenous 17 Rabbit IgG
IP
IHC-P
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

COX IV (3E11) Rabbit mAb detects endogenous levels of total COX IV protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

  1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
  3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
  4. Barrientos, A. et al. (2002) Gene 286, 53-63.

Application References

  • Aschrafi, A. et al. (2008) J Neurosci 28, 12581-90. Applications: Western Blotting.
  • Obexer, P. et al. (2009) J Biol Chem 284, 30933-40. Applications: Western Blotting.
  • Nagata, N. et al. (2012) Endocrinology 153, 3158-69. Applications: Western Blotting.
  • Chacko, A.D. et al. (2010) BMC Cancer 10, 380. Applications: IF-IC (In Cells), Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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