New England Biolabs Canada
 

Product Pathways - Protein Folding

HSP60 (D307) Antibody #4870

Item# Description List Price Web Price Qty
4870S HSP60 (D307) Antibody - 100 µl $372.00
$334.80
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
12165S HSP60 (D6F1) XP® Rabbit mAb - 100 µl $419.00
$377.10
ADD TO CART
12165T HSP60 (D6F1) XP® Rabbit mAb - 20 µl $174.00
$99.00
ADD TO CART
12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
$104.40
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
$110.70
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
ADD TO CART
2723S Hip Antibody - 100 µl $372.00
$334.80
ADD TO CART
4356S HSF1 Antibody - 100 µl $372.00
$334.80
ADD TO CART
4356T HSF1 Antibody - 20 µl $159.00
$99.00
ADD TO CART
4868S HSP40 Antibody - 100 µl $372.00
$334.80
ADD TO CART
4869S HSP60 (D85) Antibody - 100 µl $372.00
$334.80
ADD TO CART
4872S HSP70 Antibody - 100 µl $372.00
$334.80
ADD TO CART
4872T HSP70 Antibody - 20 µl $159.00
$99.00
ADD TO CART
4873S HSP70 (6B3) Rat mAb - 100 µl $372.00
$334.80
ADD TO CART
4874S HSP90 Antibody - 100 µl $372.00
$334.80
ADD TO CART
4875S HSP90 (E289) Antibody - 100 µl $372.00
$334.80
ADD TO CART
4876S HSP70 (D69) Antibody - 100 µl $372.00
$334.80
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
ADD TO CART
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
ADD TO CART
7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
ADD TO CART
7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey, D. melanogaster Endogenous 60 Rabbit
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Protocols

Specificity / Sensitivity

HSP60 (D307) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp307 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using HSP60 (D307) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HSP60 (D307) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using HSP60 (D307) Antibody (blue) compared to a nonspecific negative control antibody (red).


Background

In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).

  1. Hartl, F.U. (1996) Nature 381, 571-579.
  2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
  3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
  4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
  5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
  6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
  7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
  8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites