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Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901

Item# Description List Price Web Price Qty
4901S Phospho-cdc25C (Ser216) (63F9) Rabbit mAb - 100 µl $439.00
$395.10
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4901T Phospho-cdc25C (Ser216) (63F9) Rabbit mAb - 20 µl $174.00
$156.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
1190S Phospho-cdc25C (Ser216) Blocking Peptide - 100 µg $458.00
$412.20
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12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
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12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
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3652S cdc25A Antibody - 100 µl $372.00
$334.80
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4688S cdc25C (5H9) Rabbit mAb - 100 µl $372.00
$334.80
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4688T cdc25C (5H9) Rabbit mAb - 20 µl $159.00
$143.10
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6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
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6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
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8112L SignalStain® Antibody Diluent - 100 ml $117.00
$105.30
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8112S SignalStain® Antibody Diluent - 25 ml $57.00
$51.30
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
$50.40
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8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $525.00
$472.50
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9525S cdc25B Antibody - 100 µl $372.00
$334.80
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9527S Phospho-cdc25C (Thr48) Antibody - 100 µl $433.00
$389.70
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9527T Phospho-cdc25C (Thr48) Antibody - 20 µl $174.00
$156.60
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9528S Phospho-cdc25C (Ser216) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9529S Phospho-cdc25C (Ser198) Antibody - 100 µl $433.00
$389.70
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Monkey Endogenous 60 Rabbit IgG
IP
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

Phospho-cdc25C (Ser216) (63F9) Rabbit mAb detects endogenous levels of cdc25C only when phosphorylated at Ser216.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C.

Western Blotting

Western Blotting

Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit Mab, #4688, (lower).

Western Blotting

Western Blotting

Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb (upper) or cdc25C antibody #9522 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide #1190 (right).

Background

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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