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Product Pathways - Protein Stability

CUL1 Antibody #4995

Item# Description List Price Web Price Qty
4995S CUL1 Antibody - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat Endogenous 90 Rabbit
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

The CUL1 Antibody detects endogenous levels of CUL1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human CUL1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, untreated or MLN4924-treated (0.3uM, 16 hours) probed with CUL1 (left), #2754 NEDD8 (19E3) Rabbit mAb (middle), or #8457 beta-Actin (D6A8) Rabbit mAb (right).

Background

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). Cullin homolog 1 (CUL1), the mammalian homolog of Cdc53 from yeast, is a molecular scaffold of the SCF (Skp1/CUL1/F-box) E3 ubiquitin ligase protein complex. Thus, CUL1 and its family members function in ubiquitin dependent proteolysis (6). In particular, CUL1 has been shown to mediate ubiquitin dependent degradation of p21 Waf1/Cip1, cyclin D and IkappaB-alpha (7,8).

  1. Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
  2. Hochstrasser, M. (2000) Nat Cell Biol. 2, E153-E157.
  3. Hochstrasser, M. (2000) Science 289, 563-564.
  4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-189.
  5. Deffenbaugh, A. E. et al. (2003) Cell 114, 611-622.
  6. Pan, Z. Q. et al. (2004) Oncogene. 23, 1985-1997.
  7. Yu, Z. K. et al. (1998) Proc Natl Acad Sci. 95, 11324-11329.
  8. Read, M. A. et al. (2000) Mol Cell Biol. 20, 2326-2333.

Application References

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