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XP Monoclonal Antibody

Product Pathways - Metabolism

GAPDH (D16H11) XP® Rabbit mAb #5174

Item# Description List Price Web Price Qty
5174S GAPDH (D16H11) XP® Rabbit mAb - 100 µl $419.00
$377.10
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5174T GAPDH (D16H11) XP® Rabbit mAb - 20 µl $167.00
$150.30
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 37 Rabbit IgG
IHC-P
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)

Homology

Species predicted to react based on 100% sequence homology: Pig.

Specificity / Sensitivity

GAPDH (D16H11) XP® Rabbit mAb detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GAPDH.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

  1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
  2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
  3. Zheng, L. et al. (2003) Cell 114, 255-66.
  4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
  5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.
  6. Kozako, T. et al. (2015) Sci Rep 5, 11345.

Application References

  • Kühne, H. et al. (2014) Lipids Health Dis 13, 51. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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