New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Angiogenesis

HGF β (D6S7D) XP® Rabbit mAb #52445

Item# Description List Price Web Price Qty
52445S HGF Beta (D6S7D) XP® Rabbit mAb - 100 µl $433.00
$389.70
ADD TO CART
52445T HGF β (D6S7D) XP® Rabbit mAb - 20 µl $184.00
$165.60
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
$104.40
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
$110.70
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
ADD TO CART
14746P SignalStain® Citrate Unmasking Solution (10X) - 25 ml $36.00
$32.40
ADD TO CART
14746S SignalStain® Citrate Unmasking Solution (10X) - 125 ml $133.00
$119.70
ADD TO CART
15019L Animal-Free Blocking Solution (5X) - 250 ml $193.00
$173.70
ADD TO CART
15019S Animal-Free Blocking Solution (5X) - 25 ml $57.00
$51.30
ADD TO CART
5425S Normal Goat Serum - 10 ml $92.00
$82.80
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
ADD TO CART
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
ADD TO CART
7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
ADD TO CART
7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
ADD TO CART
8059P SignalStain® DAB Substrate Kit - 1 Kit $60.00
$54.00
ADD TO CART
8059S SignalStain® DAB Substrate Kit - 1 Kit $200.00
$180.00
ADD TO CART
8112L SignalStain® Antibody Diluent - 100 ml $117.00
$105.30
ADD TO CART
8112S SignalStain® Antibody Diluent - 25 ml $57.00
$51.30
ADD TO CART
8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
$50.40
ADD TO CART
8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $525.00
$472.50
ADD TO CART
8198S Met (D1C2) XP® Rabbit mAb - 100 µl $433.00
$389.70
ADD TO CART
8198T Met (D1C2) XP® Rabbit mAb - 20 µl $184.00
$99.00
ADD TO CART
9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $90.00
$81.00
ADD TO CART
9998S BSA - 50 g $313.00
$281.70
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 35, 85 Rabbit IgG
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

HGF β (D6S7D) XP® Rabbit mAb recognizes endogenous levels of total HGF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HGF protein.

Western Blotting

Western Blotting

Western blot analysis of recombinant human HGF (rhHGF) and extracts from A-204 and HeLa cells using HGF β (D6S7D) Rabbit mAb (upper) and β-Actin (D6A8) XP® Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast HGF β (D6S7D)

XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A-204 (left) and HeLa (right) cell pellets using HGF β (D6S7D) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human embryonal rhabdomyosarcoma using HGF β (D6S7D) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human uterine rhabdomyosarcoma using HGF β (D6S7D) XP® Rabbit mAb.

Background

The Hepatocyte Growth Factor (HGF, also known as Scatter Factor) was initially discovered as a mitogenic cytokine that induced hepatocyte replication and proliferation (1-3). HGF is produced by stromal cells where it is processed by extracellular serine proteases into a heterodimer consisting of alpha and beta subunits (4). Through activation of its receptor, cMET, HGF has a wide range of effects beyond hepatocytes that includes angiogenesis, epithelial cell proliferation and morphogenesis, and tissue protection and regeneration (5). The HGF-cMET axis has been associated with several diseases, including cancer, where HGF has been shown to promote invasion, metastasis, and drug resistance (6,7). These research studies suggest that HGF is a potential diagnostic and therapeutic target.

  1. Nakamura, T. et al. (1984) Biochem Biophys Res Commun 122, 1450-9.
  2. Russell, W.E. et al. (1984) J Cell Physiol 119, 183-92.
  3. Gohda, E. et al. (1988) J Clin Invest 81, 414-9.
  4. Kataoka, H. et al. Cancer Metastasis Rev 22, 223-36.
  5. Nakamura, T. and Mizuno, S. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86, 588-610.
  6. Matsumoto, K. and Nakamura, T. (2006) Int J Cancer 119, 477-83.
  7. Yano, S. et al. (2008) Cancer Res 68, 9479-87.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites