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Product Pathways - Cytoskeletal Signaling

COX IV (3E11) Rabbit mAb (HRP Conjugate) #5247

Item# Description List Price Web Price Qty
5247S COX IV (3E11) Rabbit mAb (HRP Conjugate) - 100 µl $436.00
$392.40
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Rat, Monkey, Zebrafish, Bovine, Pig Endogenous 17 Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

COX IV (3E11) Rabbit mAb (HRP Conjugate) detects endogenous levels of total COX IV protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, HeLa and COS cell lines, using COX IV (3E11) Rabbit mAb (HRP Conjugate).

Description

This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (COX IV (3E11) Rabbit mAb #4850).

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

  1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
  3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
  4. Barrientos, A. et al. (2002) Gene 286, 53-63.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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