New England Biolabs Canada
 

Product Pathways - Apoptosis

Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284

Item# Description List Price Web Price Qty
5284L Phospho-Bad (Ser112) (40A9) Rabbit mAb - 300 µl $1,028.00
$925.20
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5284S Phospho-Bad (Ser112) (40A9) Rabbit mAb - 100 µl $439.00
$395.10
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5284T Phospho-Bad (Ser112) (40A9) Rabbit mAb - 20 µl $174.00
$156.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
1026S Phospho-Bad (Ser112) Blocking Peptide - 100 µg $458.00
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12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
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12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
$110.70
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
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5286S Phospho-Bad (Ser136) (185D10) Rabbit mAb - 200 µl $439.00
$395.10
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5286T Phospho-Bad (Ser136) (185D10) Rabbit mAb - 20 µl $174.00
$156.60
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5425S Normal Goat Serum - 10 ml $92.00
$82.80
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6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
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6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
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8223S PhosphoPlus® Bad (Ser112) Antibody Duet - 1 Kit $714.00
$642.60
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9239S Bad (D24A9) Rabbit mAb - 100 µl $372.00
$334.80
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9239T Bad (D24A9) Rabbit mAb - 20 µl $159.00
$143.10
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9268S Bad (11E3) Rabbit mAb (IP Preferred) - 100 µl $372.00
$334.80
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9292S Bad Antibody - 100 µl $372.00
$334.80
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9292T Bad Antibody - 20 µl $159.00
$143.10
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9293S Bad Control Proteins - 100 µl $163.00
$146.70
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9296S Phospho-Bad (Ser112) (7E11) Mouse mAb - 200 µl $433.00
$389.70
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9296T Phospho-Bad (Ser112) (7E11) Mouse mAb - 20 µl $174.00
$156.60
ADD TO CART
9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $90.00
$81.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 23 Rabbit IgG
IHC-P
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry

Specificity / Sensitivity

Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase treated (right), using Phospho-Bad (Ser 112) (40A9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded COS cells untreated (left) or TPA-treated (right), showing induced cytoplasmic staining using Phospho-Bad (Ser112) (40A9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Bad (Ser 112) (40A9) Rabbit Monoclonal Antibody preincubated with control peptide (left) or Phospho-Bad (Ser 112) Blocking Peptide (IHC Specific) #1026 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, untreated (blue) or TPA/Calyculin A treated (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb.


Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Application References

  • Beharry, Z. et al. (2009) Mol Cancer Ther 8, 1473-83. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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