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Product Pathways - Growth Factors/Cytokines

GM-CSF (E8I1V) Rabbit mAb #56712

Item# Description List Price Web Price Qty
56712S GM-CSF (E8I1V) Rabbit mAb - 100 µl $364.00
$327.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 15, 18, 22 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

GM-CSF (E8I1V) Rabbit mAb recognizes endogenous levels of total GM-CSF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of human GM-CSF protein.

Western Blotting

Western Blotting

Western blot analysis of recombinant Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 using GM-CSF (E8I1V) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with TPA #4174 (80 nM, 5 hr), Ionomycin #9995 (3 μM, 5 hr), and Brefeldin A #9972 (300 ng/mL, last 4 hr of stimulation), using GM-CSF (E8I1V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

GM-CSF is produced by activated T cells, NK cells and macrophages (1,5). Target cells include granulocyte, monocyte precursors and subsets of differentiated myeloid cells (1,2,3). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in hematopoietic differentiation of dendritic cells, and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (4). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5, and PI3K/Akt pathways (1).

  1. Guthridge, M.A. et al. (1998) Stem Cells 16, 301-13.
  2. Sonoda, Y. et al. (1988) Proc Natl Acad Sci U S A 85, 4360-4.
  3. Sonoda, Y. et al. (1988) Blood 72, 1381-6.
  4. de la Cruz-Merino, L. et al. (2008) Oncologist 13, 1246-54.
  5. Zhang, A.L. et al. (2007) Blood 110, 2484-93.

Application References

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