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Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) #59997

Item# Description List Price Web Price Qty
59997S Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) - 250 µl $279.00
$251.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been affinity purified and shown no reactivity against non-immunoglobulin mouse serum proteins or bovine, goat, human, rabbit or rat IgG based on immunoelectrophoresis (IEP) testing.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using β-Actin (8H10D10) Mouse mAb #3700 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) (solid line) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line).

Description

Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.

Directions For Use

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.

Background

This product has been optimized for use as a secondary antibody in flow cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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