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Product Pathways - siRNA

SignalSilence® Control siRNA (Fluorescein Conjugate) #6201

Item# Description List Price Web Price Qty
6201S SignalSilence® Control siRNA (Fluorescein Conjugate) - 60 µl $166.00
$149.40
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Applications Reactivity
Transfection

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, either untransfected or transfected with NF-κB p65 siRNA #6261 or SignalSilence® Control siRNA (Fluorescein Conjugate), using NF-κB p65 Antibody #3034.

Fluorescent Detection

Fluorescent Detection

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. TransIT-TKO® Transfection Reagent from Mirus was used (Mirus catalog #MIR2114).

Description

RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. SignalSilence® Control siRNA (Fluorescein Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is fluorescein-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.

Directions For Use

CST recommends transfection with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) 24 to 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 20 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 µl per well.

Application References

  • Dai, Y. et al. (2004) . Journal Of Biological Chemistry 279, 34227-34239. Applications: siRNA.

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