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Product Pathways - siRNA

SignalSilence® Control siRNA (Unconjugated) #6568

Item# Description List Price Web Price Qty
6568S SignalSilence® Control siRNA (Unconjugated) - 150 µl $166.00
$149.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
6201S SignalSilence® Control siRNA (Fluorescein Conjugate) - 60 µl $166.00
$149.40
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Applications Reactivity
Transfection

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells 48 hours following transfection with 100 nM non-targeted SignalSilence® Control siRNA (Unconjugated). Protein expression levels are measured using Akt (pan) (C67E7) Rabbit mAb #4691 (left), β-Actin (13E5) Rabbit mAb #4970 (middle) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (right).

Description

RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. The introduction of a specific small interfering RNA (siRNA) strand prevents the translation of a target protein message through RNA degradation. SignalSilence® Control siRNA (Unconjugated) is a siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. This duplex contains the same scrambled sequence used in SignalSilence® Control siRNA (Fluorescein Conjugate) #6201.

Directions For Use

CST recommends transfection with 100 nM Control siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 50 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Application References

  • Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: siRNA.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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