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CD200 (E2K4C) Rabbit mAb #69858

Item# Description List Price Web Price Qty
69858S CD200 (E2K4C) Rabbit mAb - 100 µl $364.00
$327.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 45-50 Rabbit IgG
IP
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry

Specificity / Sensitivity

CD200 (E2K4C) Rabbit mAb recognizes endogenous levels of total CD200 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human CD200 recombinant protein and reacts with an epitope surrounding Pro189.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CD200 (E2K4C) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Immunoprecipitation of CD200 from SK-MEL-28 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD200 (E2K4C) Rabbit mAb. Western blot analysis was performed using CD200 (E2K4C) Rabbit mAb as the primary antibody and Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 as the secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of DU 145 cells (blue) and SK-MEL-28 cells (green) using CD200 (E2K4C) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of live human peripheral blood mononuclear cells, using CD200 (E2K4C) Rabbit mAb and co-stained with CD19 (SJ25C1) Mouse mAb (APC Conjugate) #31943 (right), compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Cells within the lymphocyte gate were used for analysis.

Background

CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7).

  1. Barclay, A.N. (1981) Immunology 44, 727-36.
  2. McCaughan, G.W. et al. (1987) Immunogenetics 25, 329-35.
  3. Wright, G.J. et al. (2000) Immunity 13, 233-42.
  4. Hoek, R.M. et al. (2000) Science 290, 1768-71.
  5. Wright, G.J. et al. (2003) J Immunol 171, 3034-46.
  6. Kretz-Rommel, A. et al. (2007) J Immunol 178, 5595-605.
  7. Fallarino, F. et al. (2004) J Immunol 173, 3748-54.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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