New England Biolabs Canada
 

Product Pathways - Companion Products

Anti-biotin, HRP-linked Antibody #7075

Item# Description List Price Web Price Qty
7075S Anti-biotin, HRP-linked Antibody - 1 ml $189.00
$170.10
$141.75
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $319.00
$287.10
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $90.00
$81.00
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS

Protocols

Specificity / Sensitivity

This antibody may cross react with endogenously expressed biotinylated proteins. Careful titration may be needed.

Description

Affinity purified goat anti-biotin antibody is conjugated to horseradish peroxidase. This product has been optimized to detect biotinylated protein markers.

Directions For Use

For western blots, incubate membrane and diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween(R) 20 at room temperature for one hour prior to detection. Note: Sodium azide is an inhibitor of HRP-binding and should not be included in the dilution buffer.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites